ELISA Rat Latrophilin-3 (LPHN3)

Reactivity:Rat (Rattus norvegicus) UniProt:Q9Z173 Abbreviation:LPHN3 Alternative Names:CIRL3; LEC3; calcium-independent alpha-latrotoxin receptor 3|latrophilin homolog 3 (cow)|lectomedin 3 Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.114 ng/mL Intra-AssayCV:?5.4% Inter-AssayCV:?8.3% Recovery:1.03 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate LPHN3 in samples. An antibody specific for LPHN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPHN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LPHN3 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPHN3 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Latrophilin-3 is a member of the latrophilin subfamily of G-protein coupled receptors (GPCR). Latrophilins may function in both cell adhesion and signal transduction. In experiments with non- species, endogenous proteolytic cleavage within a cysteine-rich GPS (G-protein-coupled-receptor proteolysis site) domain resµLted in two subunits (a large extracellµLar N-terminal cell adhesion subunit and a subunit with substantial similarity to the secretin/calcitonin family of GPCRs) being non-covalently bound at the cell membrane. Latrophilin 3forms a heterodimer, consisting of a large extracellµLar region (p120) non-covalently linked to a seven-transmembrane moiety (p85) Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).