ELISA Rat Cross linked C-telopeptide of type I collagen (CTX-I)

Reactivity:Rat (Rattus norvegicus) UniProt:N/A Abbreviation:CTX-I Alternative Names:N/A Application:ELISA Range:123.5-10000 pg/mL Sensitivity:48.1 pg/mL Intra-AssayCV:?4.5% Inter-AssayCV:?8.5% Recovery:0.98 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate CTX-I in samples. An antibody specific for CTX-I has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCTX-I present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for CTX-I is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTX-I bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The carboxyterminal telopeptide of type I collagen (ICTP) is an indicator of degradation of type I collagen. ICTP antigen consists of a trivalent collagen cross-link joining three polypeptide chains of which two are ?1 chains of one collagen molecµLe while the third is derived from either an ?1 or an ?2 chain of the helical region of another molecµLe (Fig. 1) (Risteli et al. 1993). The major determinant for antigeneity must contain two phenylalanine-rich regions, which is only possible if the cross-link is trivalent in nature (Sassi et al. 2000). In osteoclasts, catepsin K cleaves the trivalently cross-linked ICTP structure at two sites between the phenylalanine-rich region and the cross-link, thereby destroying any reactivity with ICTP antibodies (Sassi et al. 2000). Therefore degradation of type I collagen in bone is not measurable by the present ICTP test. ICTP is cleared from circµLation by the kidneys and has a molecµLar mass of about 12 000–20 000 (Risteli et al. 1993). Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).