ELISA Phospho-Histone H2A.X (Ser139) anti-

Quantity :100µL Clone Number: Aliases:H2A histone family member X antibody; H2A histone family member X antibody; H2A.FX antibody; H2A.X antibody; H2a/x antibody; H2AFX antibody; H2AX antibody; H2AX_ antibody; Histone H2A.X antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P16104 Immunogen:Peptide sequence around phosphorylation site of serine 139 (Q-A-S(p)-Q-E) derived from Histone H2A.X. Raised in:Rabbit Reactivity: Tested Applications:ELISA, WB, IF; WB:1:500-1:1000, IF:1:100-1:200 Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellµLar machineries which require DNA as a template. Histones thereby play a central role in transcription regµLation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regµLated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. Yaneva M, et al. (2005) Nucleic Acids Res. 33(16): 5320-5330. Tsukuda T, et al.(2006) Nature. Author manuscript; available in PMC 2006 March 6. Clonality:Polyclonal Isotype:IgG Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjµgates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi Conjµgate:Non-conjµgated Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:H2AFX Research Areas:Epigenetics and Nuclear Signaling